Table 3.
Growth phenotypes of bauRABCD mutants on polyamines, β-alanine, and GABA
| Strain | Genotype | Growth with various supplementsa |
||||||
|---|---|---|---|---|---|---|---|---|
| Glu | Spd | Cad | Put | DAP | β-Ala | GABA | ||
| PAO1 | Wild type | +++ | +++ | +++ | +++ | + | +++ | +++ |
| ΔbauD::GmΩ | +++ | +++ | +++ | +++ | + | ++ | +++ | |
| ΔbauC::GmΩ | +++ | ++ | +++ | +++ | − | − | +++ | |
| ΔbauB::GmΩ | +++ | + | +++ | +++ | − | − | +++ | |
| ΔbauA::GmΩ | +++ | + | +++ | +++ | − | − | +++ | |
| ΔbauR::GmΩ | +++ | + | +++ | +++ | − | − | +++ | |
| MPAO1 | Wild type | +++ | +++ | ND | +++ | + | +++ | +++ |
| bauD::IS | +++ | +++ | ND | +++ | + | ++ | +++ | |
| bauC::IS | +++ | + | ND | +++ | − | − | +++ | |
| bauA::IS | +++ | ++ | ND | +++ | − | − | +++ | |
| bauR::IS | +++ | ++ | ND | +++ | − | − | +++ | |
The cells were grown in minimal medium with the indicated supplements as the sole source of carbon and nitrogen. Glu, glutamate; Spd, spermidine; Cad, cadaverine; Put, putrescine; DAP, 1, 3 diaminopropane; β-Ala, β-alanine; GABA, γ-aminobutyrate. All supplements were added to 10 mM except β-alanine to 2.5 mM. The optical density at 600 nm (OD600) of each culture (2 ml in 15-ml culture tubes) was recorded after growth for 48 h at 37°C. −, OD600 < 0.1; +, 0.1 < OD600 < 0.3; ++, 0.3 < OD600 < 0.8; +++, OD600 > 0.8; ND, not determined. Deletion mutants with a gentamicin-resistant cassette (GmΩ) were derived from PAO1, and transposon insertion (IS) mutants were derived from MPAO1 and obtained from the stock center at University of Washington.