Table 1.

Primers used in this study

Target	Primer name	Product size (bp)	Sequence (5′–3′)
kshA1	KshARho-1	1,191	F, CATATGAGCCTCGGCACTTCCGAA
			R, GCGCTAGCCCGCGGTGGTGGACT
kshA2	KshaRho-2	1,214	F2, CATATGGCACCGTGGGTTCCAC
			R, GCGTCAGGATCCCGATTCGGCGG
kshA3	KshaRho-3	1,164	F, GCGCATATGGACATGGCACAGATT
			R, TCGTCACACCTCCGCTTCCTGCTT
kshA5	KshaRho-5	1,179	F, CATATGTCCATCGACACCGCACG
			R, GCTCTAGGGGGTCGCGGTGGAGC
kshA1	KshA1Rho-RT	374	F, AACGGCCGCTGCAAGAACATC
			R1, TTGACCGTTCGCGTGCGGAC
kshA2	KshA2Rho-RT	338	F2, CCGTTCCGGAGGTTCCCACC
			R2, TCGGCGTCGACTCGGGATCA
kshA3	KshA3Rho-RT	371	F, AACGGCAAGTGCACGGACATC
			R1, CTGCGTCGCCATGCCCTTGT
kshA4	KshA4Rho-RT	333	F1, CGAAGCTCGGCCGTACCAAG
			R1, ATCTGCACACCGGTCTTGAT
kshA5	KshA5Rho-RT	407	F, GCCTGGACGACCCTCGAACGC
			R1, GTCTGGCCGTTGGCATCGGA
16S RNA	16SF167Ameth	350	GTGGCCTACCAAGGCGACGA
	16SR483Ameth		AGACGCGACAAGCCGCCTAC