Table 5.
Suppression of the His relocation mutants by ExbB(A39E)
| Mutanta | Sensitivity tob: |
||||
|---|---|---|---|---|---|
| Colicin B | Colicin D | Colicin M | Colicin Ia | φ80 | |
| Chromosomal | 9, 9, 9 | 7, 7, 7 | 5, 4, 5 | 9, 9, 8 | 8, 8, 8 |
| pKP325 (wild type) | 8, 8, 8 | 6, 6, 5 | 5, 5, 5 | 6, 7, 7 | 8, 8, 8 |
| ΔtonB | T, T, T | T, T, T | T, T, T | T, T, T | T, T, T |
| ΔV17 | 4*, 4*, 4* | 3, 3, 3 | T, T, T | T, T, T | 3, 3, 5 |
| P12H H20A | T, T, T | T, T, T | T, T, T | 2*, 2*, 2* | 6, 5, 6 |
| T13H H20A | T, T, T | T, T, T | T, T, T | T, T, T | 3, 4, 5 |
| L14H H20A | 4*, 4*, 4* | 3, 2, 3 | T, T, T | 4, 4, 3 | 7, 6, 6 |
| L15H H20A | T, T, T | T, 2*, T | T, T, T | 2, 2, 3 | 5, 5, 6 |
| S16H H20A | T, 1*, 3* | T, T, T | T, T, T | T, T, T | 7, 6, 6 |
| V17H H20A | T, T, T | T, T, T | T, T, T | 2*, T, T | 7, 5, 7 |
| C18H H20A | 5*, 2*, 3* | 1*, 2*, 1* | T, T, T | 2*, 3*, 1* | 6, 6, 7 |
| I19H H20A | T, T, T | T, T, T | T, T, T | T, T, T | 5, 5, 6 |
| G21H H20A | 5, 5, 6 | 2*, 2*, 2* | 4, 3, 3 | 4, 4, 3 | 7, 8, 8 |
| A22H H20A | 3*, 4*, 5* | 2, 2, 3 | T, T, T | 4, 5, 5 | 4, 4, 5 |
| V23H H20A | T, T, T | T, T, T | T, T, T | 3, 3, 3 | 6, 7, 7 |
| V24H H20A | T, T, T | T, T, T | T, T, T | T, T, T | 3, 3, 4 |
| A25H H20A | 7, 7, 7 | 4, 5, 5 | 2, 2, 3 | 6, 6, 6 | 7, 8, 9 |
| G26H H20A | T, T, T | 2, 1, 1 | T, T, T | 5, 5, 6 | 4, 4, 5 |
| L27H H20A | 5, 5, 6 | 2, 3, 3 | T, T, T | 3, 4, 4 | 5, 5, 6 |
| L28H H20A | 5, 5, 5 | T, T, T | 3, 4, 4 | 4, 4, 4 | 7, 8, 8 |
| Y29H H20A | 3, 4, 5 | 2, 3, 2 | T, T, T | 3, 4, 5 | 7, 8, 8 |
| T30H H20A | T, T, T | 1*, 1*, 1* | T, T, T | 2*, 2*, 3* | 7, 7, 8 |
| S31H H20A | T, T, T | T, T, T | T, T, T | T, T, T | 5, 5, 5 |
| V32H H20A | 5, 6, 5 | 3, 3, 3 | 2, 2, T | T, T, T | 3, 3, 3 |
a
Chromosomal indicates the wild-type strain W3110 carrying the vector plasmid pKP477. ΔtonB indicates strain KP1353 [tonB aroB exbB(A39E)] which served as the background in which all plasmids were analyzed. Wild-type TonB from pKP325 and TonB substitution mutants were expressed at chromosomal levels using the l-arabinose levels shown in Table 1.
b
Colicins were spotted in 5-fold dilutions in λ-Ca2+ buffer and bacteriophage φ80 was spotted in 10-fold dilutions in λ-Ca2+ buffer. Results of triplicate assays for the highest dilution at which clearing was observed are shown. T, tolerance (insensitivity) to the agent tested; *, clearing was faint at all dilutions.