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Characterization of the T. denticola ΔtbpA mutant. (a) Western blotting of the T. denticola ΔtbpA mutant. The same amounts of wild-type T. denticola and T. denticola ΔtbpA whole lysates were analyzed by SDS-PAGE. The immunoblotting was conducted as described for Fig. 5b. (b) RT-PCR analysis of the T. denticola ΔtbpA mutant. The transcripts of TDE0144 and TDE0145, the downstream genes of tbpATd, in wild-type T. denticola and T. denticola ΔtbpA were detected by RT-PCR.
