Table 1.
Primer | Sequence (5′ → 3′)a | Commentb |
---|---|---|
P1 | GACAGTTTACTCGGCACG | Flanking region before TDE0143; F |
P2 | ATCGATCAGCCCTTTGCGGGTATG | Flanking region before TDE0143; R |
P3 | ATCGATCTCAAAGCCTCCTTCCCG | Flanking region after TDE0143; F |
P4 | AGATATTTCGGTGAGGGC | Flanking region after TDE0143; R |
P5 | ATCGATCCGATAGCTTCCGCTATTGC | erm cassette; F |
P6 | ATCGATGGAAGCTGTCAGTAGTATACC | erm cassette; R |
P7 | CGGAGAAGCTCCCATAGC | Co-RT-PCR, TDE0143-TDE0144; F |
P8 | AAAGATGAGGCCGCCGAG | Co-RT-PCR, TDE0143-TDE0144; R |
P9 | CCCATGTGCAAGAAGGGC | Co-RT-PCR, TDE0144-TDE0145; F |
P10 | GACCATTCCGATTCCGCG | Co-RT-PCR, TDE0144-TDE0145; R |
P11 | CATTACCCCTCTTGTCGAG | Co-RT-PCR, TDE0145-TDE0146; F |
P12 | CGTTAATCTCGGCTATACC | Co-RT-PCR, TDE0145-TDE0146; R |
P13 | CACCTGTGCCGCTCTTTTTGCA | rTbpATd; F |
P14 | CTAAGCAAGTCCCTTGATAAC | rTbpATd; R |
P15 | GAATTCGGACAACTTGTTATTATGCAC | 5′ of tbpATd thi-box; F |
P16 | GGATCCTGCAAAAAGAGCGGCACAACC | 3′ of tbpATd thi-box-L; R |
P17 | GGATCCCATATGCTCCCTCCGCCGGTA | 3′ of tbpATd thi-box-S; R |
P18 | TAATTATCGGTTGTGCCGCT | TDE0143 5′-RACE sensor primer; F |
P19 | CGGCAATACCCATACCTTCA | TDE0143 5′-RACE inner primer; R |
P20 | TGCAGGTTTGGGAACATCCT | TDE0143 5′-RACE outer primer; R |
P21 | CTGACGGAACAAAGGTTGTAG | qRT-PCR, TDE0143; F |
P22 | CCTTACCTTCCAGTATGACTC | qRT-PCR, TDE0143; R |
P23 | TAGGATTGCTGCCTTTACGG | qRT-PCR, TDE0144; F |
P24 | ATTATTGCAGGCACGCAGAG | qRT-PCR, TDE0144; R |
P25 | TCAGGCTGCGGCAAAACAAC | qRT-PCR, TDE0145; F |
P26 | CATTGAGATGAGGGAAGAGG | qRT-PCR, TDE0145; R |
P27 | CCGTCCATTGTAGGCTTTAC | qRT-PCR, dnaK; F |
P28 | CCGTCAATGTCGATTCGTAC | qRT-PCR, dnaK; R |
P29 | TGCTTTTACAAGCTAAAAACATACCCGCAA | TPP-binding site mutagenesis; F |
P30 | TTGCGGGTATGTTTTTAGCTTGTAAAAGCA | TPP-binding site mutagenesis; R |
P31 | GCAGCACATCCCCCTTTC | qRT-PCR, lacZ; F |
P32 | CGTTGGTGTAGATGGGCG | qRT-PCR, lacZ; R |
P33 | AACGTACTGGCAGATGCAG | qRT-PCR, groEL; F |
P34 | GCGCTTTCAGTTCTTCAACTG | qRT-PCR, groEL; R |
The engineered restriction enzyme sites are underlined.
F, forward primer; R, reverse primer.