Table 1.
Oligonucleotide primers used in this study
| Primer | Sequence (5′ → 3′)a | Commentb |
|---|---|---|
| P1 | GACAGTTTACTCGGCACG | Flanking region before TDE0143; F |
| P2 | ATCGATCAGCCCTTTGCGGGTATG | Flanking region before TDE0143; R |
| P3 | ATCGATCTCAAAGCCTCCTTCCCG | Flanking region after TDE0143; F |
| P4 | AGATATTTCGGTGAGGGC | Flanking region after TDE0143; R |
| P5 | ATCGATCCGATAGCTTCCGCTATTGC | erm cassette; F |
| P6 | ATCGATGGAAGCTGTCAGTAGTATACC | erm cassette; R |
| P7 | CGGAGAAGCTCCCATAGC | Co-RT-PCR, TDE0143-TDE0144; F |
| P8 | AAAGATGAGGCCGCCGAG | Co-RT-PCR, TDE0143-TDE0144; R |
| P9 | CCCATGTGCAAGAAGGGC | Co-RT-PCR, TDE0144-TDE0145; F |
| P10 | GACCATTCCGATTCCGCG | Co-RT-PCR, TDE0144-TDE0145; R |
| P11 | CATTACCCCTCTTGTCGAG | Co-RT-PCR, TDE0145-TDE0146; F |
| P12 | CGTTAATCTCGGCTATACC | Co-RT-PCR, TDE0145-TDE0146; R |
| P13 | CACCTGTGCCGCTCTTTTTGCA | rTbpATd; F |
| P14 | CTAAGCAAGTCCCTTGATAAC | rTbpATd; R |
| P15 | GAATTCGGACAACTTGTTATTATGCAC | 5′ of tbpATd thi-box; F |
| P16 | GGATCCTGCAAAAAGAGCGGCACAACC | 3′ of tbpATd thi-box-L; R |
| P17 | GGATCCCATATGCTCCCTCCGCCGGTA | 3′ of tbpATd thi-box-S; R |
| P18 | TAATTATCGGTTGTGCCGCT | TDE0143 5′-RACE sensor primer; F |
| P19 | CGGCAATACCCATACCTTCA | TDE0143 5′-RACE inner primer; R |
| P20 | TGCAGGTTTGGGAACATCCT | TDE0143 5′-RACE outer primer; R |
| P21 | CTGACGGAACAAAGGTTGTAG | qRT-PCR, TDE0143; F |
| P22 | CCTTACCTTCCAGTATGACTC | qRT-PCR, TDE0143; R |
| P23 | TAGGATTGCTGCCTTTACGG | qRT-PCR, TDE0144; F |
| P24 | ATTATTGCAGGCACGCAGAG | qRT-PCR, TDE0144; R |
| P25 | TCAGGCTGCGGCAAAACAAC | qRT-PCR, TDE0145; F |
| P26 | CATTGAGATGAGGGAAGAGG | qRT-PCR, TDE0145; R |
| P27 | CCGTCCATTGTAGGCTTTAC | qRT-PCR, dnaK; F |
| P28 | CCGTCAATGTCGATTCGTAC | qRT-PCR, dnaK; R |
| P29 | TGCTTTTACAAGCTAAAAACATACCCGCAA | TPP-binding site mutagenesis; F |
| P30 | TTGCGGGTATGTTTTTAGCTTGTAAAAGCA | TPP-binding site mutagenesis; R |
| P31 | GCAGCACATCCCCCTTTC | qRT-PCR, lacZ; F |
| P32 | CGTTGGTGTAGATGGGCG | qRT-PCR, lacZ; R |
| P33 | AACGTACTGGCAGATGCAG | qRT-PCR, groEL; F |
| P34 | GCGCTTTCAGTTCTTCAACTG | qRT-PCR, groEL; R |
a
The engineered restriction enzyme sites are underlined.
b
F, forward primer; R, reverse primer.