Table 4.
TPP concn in TA medium | Mean intracellular TPP concn (nM) ± SEM in: |
|
---|---|---|
Wild-type T. denticola | ΔtbpA mutant | |
100 nM | 94.43 ± 8.96 | |
10 μM | 184.85 ± 4.52 | 94.49 ± 6.97 |
50 μM | 258.39 ± 7.21 | 230.34 ± 4.3 |
The two strains were cultivated in TA medium containing different concentrations of TPP (100 nM, 10 μM, and 50 μM). Three independent assays were conducted, and the intracellular concentrations of TPP were determined. The ΔtbpA mutant failed to grow at the low concentration of TPP (100 nM).