Fig. 8.

Peptidoglycan-hydrolyzing activity of MBP-VvpS. MBP-VvpS protein (100 μM) was used for determination of PGN-hydrolyzing activity. PGN-hydrolyzing activity was determined using insoluble PGNs of V. vulnificus (A) and S. aureus (B) as substrates, defined as an activity of hydrolytic solubilization of the PGNs, and measured as a decrease in the A₄₅₀ in the presence of either 1 mM PMSF or 10 mM EDTA. The same amount of MBP protein was used as a negative control. The error bars represent the SEM.