Fig. 5.
Overproduction of PomBDΔL impairs cell growth. (A) NMB191 cells harboring the plasmid pHFAB (•), pLSK9 (□), or pLSK14 (○) were cultured in VPG500 broth at 30°C for 7 h with 0.2% arabinose added at the beginning (at 0 h). Cell growth was monitored at an absorbance of 660 nm every h. (B) NMB191 cells harboring the plasmid pHFAB (•), pTSK37 (▪), or pTSK37 D24N (▴) were cultured in VPG500 broth at 30°C for 7 h with 0.2% arabinose added at the beginning (at 0 h). Cell growth was monitored at an absorbance of 660 nm every h. (C) NMB200 cells harboring the plasmid pTSK37 (▪) or pTSK37 D24N (•) were cultured at 30°C for 7 h with 0.2% arabinose added at the beginning. (D) Effect of mutations at the putative helix of the N-terminal PEM region on growth impairment. Strains with pHFAB (•), pTSK37 (▪), or pTSK37 L168P (▴) were cultured in VPG500 broth at 30°C for 7 h with 0.2% arabinose added at the beginning.