Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Descriptiona | Source or reference |
|---|---|---|
| Strains | ||
| V. alginolyticus | ||
| VIO5 | Rifr Pof+ Laf− | 30 |
| NMB191 | Rifr Pof+ Laf− ΔpomAB | 46 |
| NMB200 | RIfr Pof+ Laf− ΔpomB ΔfliG | N. Nonoyama |
| E. coli | ||
| BL21(DE3) | Host for overexpression from the T7 promoter | |
| DH5α | Recipient for cloning experiments | |
| Plasmids | ||
| pET19b | T7 expression vector | Novagen |
| pBAD33 | Cmr PBAD | 12 |
| pTY57 | Cmr PBAD with a multicloning site of pBAD24 | T. Yorimitsu |
| pSU41 | Kmr PlaclacZα | 2 |
| pAS101 | pSU41/MotY | 21 |
| pHFAB | pBAD33/PomA+PomB | 11 |
| pTSK32 | pET19b/PomBC1-His6 | This study |
| pTSK33 | pET19b/PomBC2-His6 | This study |
| pTSK34 | pET19b/PomBC3-His6 | This study |
| pTSK35 | pET19b/PomBC4-His6 | This study |
| pTSK36 | pET19b/PomBC5-His6 | This study |
| pLSK5 | pTY57/MotYL::PomBC4-His6a | This study |
| pLSK6 | pTY57/PomBC4-His6 | This study |
| pLSK7 | pTY57/MotYL::PomBC5-His6a | This study |
| pLSK8 | pTY57/PomBC5- His6 | This study |
| pLSK9 | pBAD33/PomA+PomBΔL(Δ41-60) | This study |
| pLSK10 | pBAD33/PomA+PomBΔL(Δ41-70) | This study |
| pLSK11 | pBAD33/PomA+PomBΔL(Δ41-80) | This study |
| pLSK12 | pBAD33/PomA+PomBΔL(Δ41-90) | This study |
| pLSK13 | pBAD33/PomA+PomBΔL(Δ41-100) | This study |
| pLSK14 | pBAD33/PomA+PomBΔL(Δ41-110) | This study |
| pTSK37 | pBAD33/PomA+PomBΔL(Δ41-120) | This study |
| pTSK38 | pBAD33/PomA+PomBΔL(Δ41-130) | This study |
| pTSK39 | pBAD33/PomA+PomBΔL(Δ41-140) | This study |
| pTSK37-D24N | pBAD33/PomA+PomBΔL(Δ41-120, D24N) | This study |
| pTSK37-M153P | pBAD33/PomA+PomBΔL(Δ41-120, M153P) | This study |
| pTSK37-M157P | pBAD33/PomA+PomBΔL(Δ41-120, M157P) | This study |
| pTSK37-L160P | pBAD33/PomA+PomBΔL(Δ41-120, L160P) | This study |
| pTSK37-I164P | pBAD33/PomA+PomBΔL(Δ41-120, I164P) | This study |
| pTSK37-L168P | pBAD33/PomA+PomBΔL(Δ41-120, L168P) | This study |
a
Each PomB fragment was fused to a MotY leader sequence (MotYL). Rifr, rifampin resistant; Cmr, chloramphenicol resistant; Pof+, normal polar flagellar formation, Laf−, defective in lateral flagellar formation; PBAD, arabinose promoter.