Table 3.
Motile fraction of cells expressing PomBΔL(Δ41-120) with mutations in the putative PEM region
| Strain | Percentage of motile cellsa (no. of cells analyzed) |
|---|---|
| pHFAB | 26 (358) |
| pTSK37 | 1 (265) |
| pTSK37 M153P | 2 (180) |
| pTSK37 M157P | 0 (316) |
| pTSK37 L160P | 4 (224) |
| pTSK37 I164P | 4 (283) |
| pTSK37 L168P | 10 (225) |
a
The NMB191 strain containing each plasmid was cultured at 30°C in VPG500 broth for 4 h with 0.2% arabinose, and swimming motility was observed using dark-field microscopy. Motile fractions were measured as described in Materials and Methods.