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Complementation of nusA(R258C) mutant by minimal nusA+ plasmid. The nusA(R258C) mutant GJ10524 carrying plasmid pHYD2556 (Para-nusA+) was plated at a suitable dilution on Trp- or Anth-supplemented plates with either glucose (Glu) or glycerol at 0.4% and l-arabinose at 0.2% (Gly Ara), as indicated.
