Table 1.
E. coli K-12 strains
| Straina | Genotypeb |
|---|---|
| MC4100 | Δ(argF-lac)U169 rpsL150 relA1 araD139 flbB5301 deoC1 ptsF25 |
| RS445 | MC4100 galEp3, λRS88 lysogen carrying Plac–lacZYA |
| GJ3119 | MC4100 nusA1 |
| GJ5147 | MC4100 galEp3, λRS45 lysogen carrying Plac-H-19BtR1-lacZYA |
| GJ6504 | MG1655 lacI lacZU118 trpR55 trpE9777 |
| GJ6509 | GJ6504 rho(A243E) |
| GJ6511 | GJ6504 nusG(G146D) |
| GJ6520 | GJ6509 Δ(yefM-yoeB)::Cm |
| GJ6524 | GJ6504 nusG(G146D) Δ(yefM-yoeB)::Cm |
| GJ7360 | GJ6520 argE55::Tn10 |
| GJ7361 | GJ6520 ilv-500::Tn10 |
| GJ7362 | GJ6524 ilv-500::Tn10 |
| GJ7363 | GJ6524 argE55::Tn10 |
| GJ7368c | GJ6504 Δ(yefM-yoeB)::Cm rho(R102S, A243E) zif-909::Tn10dTet/pLG-H-NSΔ64 |
| GJ7376* | GJ6524 rho(Q32R)/pLG-H-NSΔ64 |
| GJ7387* | GJ6524 nusA(R258C)/pLG-H-NSΔ64 |
| GJ7395*† | GJ7387 Cms |
| GJ7417‡ | GJ6509 nusA(R258C) truB::Tn10dCm |
| GJ7419‡ | GJ6504 nusG(G146D) nusA(R258C) truB::Tn10dCm |
| GJ10524 | GJ6504 nusA(R258C) |
| GJ10557 | GJ6524 rho(Q32R) |
| GJ10572 | GJ5147 nusA(R258C) |
| GJ10573 | RS445 nusA(R258C) |
| GJ10581‡ | GJ6504 rho(Q32R) nusA(R258C) Δ(yefM-yoeB)::Cm |
| GJ10591‡ | GJ6524 rho(A243E) |
| GJ10668d | GJ7419 rho(A243E) |
| GJ10669‡ | GJ7419 rho(Q32R) |
| GJ10697 | GJ10527 rpoB8 btuB::Tn10 |
| GJ10699 | rpoB8 btuB::Tn10 |
| GJ10700 | btuB::Tn10 |
| GJ10702 | GJ10527 btuB::Tn10 |
| GJ10753† | GJ6504 rho(Q32R) Δ(yefM-yoeB) |
| GJ10793§ | GJ10572 rho(R102S, A243E) zif-909::Tn10dTet |
| GJ10794§ | GJ10573 rho(R102S, A243E) zif-909::Tn10dTet |
| GJ10795 | GJ5147 rho(Q32R) |
| GJ10796 | GJ5147 nusG(G146D) rho(Q32R) |
| GJ10797 | GJ10572 rho(Q32R) |
| GJ10798 | RS445 rho(Q32R) |
| GJ10799 | RS445 nusG(G146D) rho(Q32R) |
| GJ10800 | GJ10573 rho(Q32R) |
| GJ10801 | GJ5147 rho(R102S, A243E) zif-909::Tn10dTet |
| GJ10802 | RS445 rho(R102S, A243E) zif-909::Tn10dTet |
| GJ10811§ | GJ10572 nusG(G146D) argE55::Tn10 |
| GJ10812§ | GJ10573 nusG(G146D) argE55::Tn10 |
| GJ10813§ | GJ10572 rho(A243E) ilv-500::Tn10 |
| GJ10814§ | GJ10573 rho(A243E) ilv-500::Tn10 |
| GJ10817§ | GJ10799 nusA(R258C) truB::Tn10dCm |
| GJ10818§ | GJ10796 nusA(R258C) truB::Tn10dCm |
Strains MC4100, RS445, GJ5147, GJ6504, GJ6509, and GJ6511 have been described in the accompanying study (47), and strain GJ3119 was described by Harinarayanan and Gowrishankar (29). Strains GJ6520 and GJ6524 were constructed by S. Aisha (unpublished); all other strains were constructed in this study. *, the indicated strain also carries a Tn10dTet (37) insertion at undetermined chromosomal locations; †, the indicated strain was constructed from its ancestor by excision of the Cmr marker in Δ(yefM-yoeB)::Cm by site-specific recombination with the aid of plasmid pCP20, as described previously (18); ‡, the indicated strain was maintained as a derivative with plasmids pHYD751 (nusG+) or pHYD1201 (rho+), as appropriate; §, the indicated strain was maintained as a derivative with plasmid pLG-H-NSΔ64.
Genotype designations are as described previously (4). All strains are F−. The rho(A243E) mutation is also interchangeably referred to as rho-4. References or sources for the mutations that were introduced by transduction into the strains are as follows: Δ(yefM-yoeB)::Cm (13); argE55::Tn10, ilv-500::Tn10, and btuB::Tn10 (50); and rpoB8 (51).
The zif-909::Tn10dTet insertion was obtained in this study and shown to be 76% cotransducible with rho.
Strain GJ10668 was maintained as a derivative with plasmids pHYD751 and pLG-H-NSΔ64.