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. 2011 Aug;193(15):3710–3721. doi: 10.1128/JB.00059-11

Fig. 3.

Fig. 3.

Modality of O-antigen polysaccharides expressed in E. coli K-12, S. enterica, and S. flexneri. Silver-stained polyacrylamide gel showing the O-antigen LPS profiles of the E. coli K-12 W3110 (lane 1) and EVV33 (ΔwzzB) isogenic strains (lanes 2, 3, 5, and 7) containing pMF19 to allow expression of O16 LPS. The LPS profiles of parental S. Typhimurium (lane 4) and S. flexneri (lane 6) are also shown. For the expression of recombinant WzzB, EVV33 also the contained pBAD vector control (lane 3) and WzzB expressing plasmids from E. coli K-12 (pWzzB; lane 2), S. Typhimurium (pWzzB-ST; lane5) and S. flexneri (pWzzB-SF; lane 7). The regions corresponding to the migration of the lipid A-core OS, long OAg (L-OAg), and very long OAg (VL-OAg) polysaccharide are indicated, as well as the average modalities of each strain (LT, S. Typhimurium; EC, E. coli K-12; and SF, S. flexneri). Note that VL-OAg is normally produced in S. Typhimurium and S. flexneri and imparted by FepE.