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. 2011 Aug;193(15):3710–3721. doi: 10.1128/JB.00059-11

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Fig. 6. — Loop 4 of Wzz is important for modality. (A) Diagrams showing the structural components of WzzB_EcO157 (WzzB; in black) and its derivative in which the entire periplasmic region was replaced with the corresponding region of FepE_EcO157 (WzzB-FepE), indicated in gray. (B) Silver-stained polyacrylamide gel showing the O-antigen LPS profile of E. coli EVV33(pMF19) containing various plasmids. Lane 1, pBAD; lane 2, pSK34 carrying the parental fepE_EcO157 (FepE); lane 3, pSK37 encoding WzzB with the FepE periplasmic domain; and lane 4, pSK38 encoding WzzB with FepE periplasmic and TM regions. (C) Diagrams showing the structural components of FepE_EcO157 (FepE; in gray) and its derivative in which parental loop 4 (L4) region was replaced with the corresponding region of WzzB_EcO157 (FepE-WzzB-L4), as indicated by the dotted line. (D) Silver-stained polyacrylamide gel showing the O-antigen LPS profile of E. coli EVV33(pMF19), with pSK34 carrying parental fepE_EcO157 (FepE; lanes 1 and 3), pSK35 carrying parental wzzB_EcO157 (WzzB; lanes 2 and 6), pSK36 carrying fepE_EcO157 (256–273 > 231–250 wzzB_EcO157) (FepE-WzzB-L4; lane 4), pSK40 carrying fepE_EcO157 with deletion of residues 256 to 273 (FepE-deltaL4; lane 5), and the pBAD24 vector control (pBAD; lane 7).