Table 1.
Strains and plasmids used in this study
| Strain/plasmid | Relevant property(ies) | Source/reference |
|---|---|---|
| Strains | ||
| DH5α | F− φ80lacZΔM15 endA recA hsdR (rK− mK−) supE thi gyrA relA Δ(lacZYA-argF)U169 | Laboratory stock |
| EVV33 | E. coli W3110 ΔwzzB | 18 |
| W3110 | rph-1 1N(rrnD-rrnE) | Laboratory stock |
| Plasmids | ||
| pBAD24 | Arabinose inducible, expression vector, Apr | 13 |
| pMF19 | wbbLEcO16 cloned into pEXT21, Spr | 8 |
| pWzzB | pEV6, parental wzzBEcK-12 cloned into pBAD24 | 37 |
| pWzzB-SF | parental wzzBSF cloned into pBAD24 | This study |
| pWzzB-ST | parental wzzBST cloned into pBAD24 | This study |
| pSK1 | wzzBST′(F95)-′wzzBSF(I96) encoding WzzB-ST-SF-T1 | This study |
| pSK2 | wzzBST′(L119)-′wzzBSF(T120) encoding WzzB-ST-SF-T2 | This study |
| pSK3 | wzzBST′(T139)-′wzzBSF(T140) encoding WzzB-ST-SF-T3 | This study |
| pSK4 | wzzBST′(Q199)-′wzzBSF(I200) encoding WzzB-ST-SF-T4 | This study |
| pSK5 | wzzBST′(Y255)-′wzzBSF(Q256) encoding WzzB-ST-SF-T5 | This study |
| pSK6 | wzzBST′(V274)-′wzzBSF(H275) encoding WzzB-ST-SF-T6 | This study |
| pSK7 | wzzBSF′(L95)-′wzzBST(I96) encoding WzzB-SF-ST-F1 | This study |
| pSK8 | wzzBSF′(L119)-′wzzBST(T120) encoding WzzB-SF-ST-F2 | This study |
| pSK9 | wzzBSF′(Q139)-′wzzBST(T140) encoding WzzB-SF-ST-F3 | This study |
| pSK10 | wzzBSF′(Q199)-′wzzBST(I200) encoding WzzB-SF-ST-F4 | This study |
| pSK11 | wzzBSF′(Y255)-′wzzBST(Q256) encoding WzzB-SF-ST-F5 | This study |
| pSK12 | wzzBSF′(I274)-′wzzBST(H275) encoding WzzB-SF-ST-F6 | This study |
| pSK13 | wzzBSF(V67-L95 >A67-F95 wzzBST) encoding WzzB-SF-T7 | This study |
| pSK14 | wzzBSF(Q202-I274 >E202-V274 wzzBST) encoding WzzB-SF-T8 | This study |
| pSK15 | wzzBSF(Q202-A253 >E202-N253 wzzBST) encoding WzzB-SF-T9 | This study |
| pSK16 | wzzBSF(Q202-K214 >E202-Q214 wzzBST) encoding WzzB-SF-T10 | This study |
| pSK17 | wzzBSF(V217-E237 >I217-K237 wzzBST) encoding WzzB-SF-T11 | This study |
| pSK18 | wzzBSF(N260-I274 >T260-V274 wzzBST) encoding WzzB-SF-T12 | This study |
| pSK19 | wzzBSF(F269-I274 >V269-V274 wzzBST) encoding WzzB-SF-T13 | This study |
| pSK20 | wzzBST(A67-F95 >V67-L95 wzzBSF) encoding WzzB-ST-F7 | This study |
| pSK21 | wzzBST(E202-V274 >Q202-I274 wzzBSF) encoding WzzB-ST-F8 | This study |
| pSK22 | wzzBST(E202-N253 >Q202-A253 wzzBSF) encoding WzzB-ST-F9 | This study |
| pSK23 | wzzBST(E202-Q214 >Q202-K214 wzzBSF) encoding WzzB-ST-F10 | This study |
| pSK24 | wzzBST(I217-K237 >I217-E237 wzzBSF) encoding WzzB-ST-F11 | This study |
| pSK25 | wzzBST(T260-V274 >N260:I274 wzzBSF) encoding WzzB-ST-F12 | This study |
| pSK26 | wzzBST(V269-V274 >F269-I274 wzzBSF) encoding WzzB-ST-F13 | This study |
| pSK27 | wzzBST(E202-Q214 >Q202-K214; T260-V274 >N260-I274 wzzBSF) encoding WzzB-ST-F14 | This study |
| pSK28 | wzzBST(A67-F95 >V67-I95; E202-Q214 >Q202-K214 wzzBSF) encoding WzzB-ST-F15 | This study |
| pSK29 | wzzBST(E202-Q214 >Q202-K214; V269-V274 > F269-I274 wzzBSF) encoding WzzB-ST-F16 | This study |
| pSK30 | wzzBSF(V67-L95 >A67:F95; F269-I274 >V269-V274 wzzBST) encoding WzzB-SF-T15 | This study |
| pSK31 | wzzBSF(V67-L95 >A67-F95; Q202-K214 >E202-Q214 wzzBST) encoding WzzB-SF-T16 | This study |
| pSK32 | wzzBSF(Q202-K214 >E202-Q214; F269-I274 >V269-V274 wzzBST) encoding WzzB-SF-T14 | This study |
| pSK33 | wzzBSF(V67-L95 >A67:F95; Q202-K214 >E202-Q214; F269-I274 >V269-V274 wzzBST) encoding WzzB-SF-T17 | This study |
| pSK34 | Parental fepEEcO157 | This study |
| pSK35 | Parental wzzBEcO157 | This study |
| pSK36 | fepEEcO157(256-273 >231-250 wzzBEcO157) | This study |
| pSK37 | wzzBEcO157(64-295 >62-338 fepEEcO157) | This study |
| pSK38 | wzzBEcO157(32-317 >42-359 fepEEcO157) | This study |
| pSK39 | wzzBST(E202-Q214 >Q202-K214; V269-V274 >F269-I274 wzzBSF) encoding WzzB-ST-F17 | This study |
| pSK40 | fepEEcO157 with deletion of residues 256 to 273 | This study |