Fig. 2.
Med acts independently of KinC and in the same pathway as KinD to promote expression of sdp. (A) Strains harbored amyE::Psdp-lacZ and were wild type (RL4264) or harbored a med mutation (AB332), Phyperspank-med (▵; AB333), a kinC mutation (AB341), a med kinC mutation (AB342), or a kinC mutation and Phyperspank-med (AB343). (B) Strains harbored amyE::Psdp-lacZ and were either wild type (RL4264) or harbored a med mutation (AB332), Phyperspank-med (AB333), a kinD mutation (AB337), a med kinD mutation (AB338), or a kinD mutation and Phyperspank-med (AB339). Cells were grown in liquid DSM; hour 0 of stationary phase was the end of the exponential phase of growth. Expression of Phyperspank-med was induced by addition of 1 mM (final concentration) IPTG to the medium. The experiment was repeated a minimum of three times. Shown are the results of a representative experiment.