Fig. 3.
Med acts independently of KinA and KinB to promote expression of sdp. (A) Strains harbored amyE::Psdp-lacZ and were either wild type (RL4264) or harbored a med mutation (AB332), Phyperspank-med (AB333), a kinA mutation (AB481), a med kinA mutation (AB482), or a kinA mutation and Phyperspank-med (AB483). (B) Strains harbored amyE::Psdp-lacZ and were either wild type (RL4264) or harbored a med mutation (AB332), Phyperspank-med (AB333), a kinB mutation (AB484), a med kinB mutation (AB485), or a kinB mutation and Phyperspank-med (AB486). Cells were grown in liquid DSM; hour 0 of stationary phase was the end of the exponential phase of growth. Expression of Phyperspank-med was induced by addition of 1 mM (final concentration) IPTG to the medium. The experiment was repeated a minimum of three times. Shown are the results of a representative experiment.