Table 1.
Strains used in this study
| Straina | Description or genotype | Reference or sourcec |
|---|---|---|
| PY79 | Prototrophic derivative of B. subtilis 168 | 41 |
| 3610b | Undomesticated wild strain capable of forming robust biofilms | 4 |
| AB142 | Δmed::erm | This study |
| AB143 | Δmed::tet | This study |
| AB307 | Δmed::tet amyE::PsdpABC-lacZ cm | AB143 x RL2886 |
| AB332 | Δmed::erm amyE::PsdpABC-lacZ spc | AB142 x RL4264 |
| AB333 | thrC::Phyperspank-med erm amyE::PsdpABC-lacZ spc | AB340 x AB340 |
| AB335 | Δmed::erm amyE::Pskf-lacZ spc | AB142 x RL3554 |
| AB336 | thrC::Phyperspank-med erm amyE::Pskf-lacZ spc | AB340 x RL3554 |
| AB337 | ΔkinD::tet amyE::PsdpABC-lacZ spc | EH456 x RL4264 |
| AB338 | ΔkinD::tet Δmed::erm amyE::PsdpABC-lacZ spc | AB337 x AB142 |
| AB339 | ΔkinD::tet thrC::Phyperspank-med erm amyE::PsdpABC-lacZ spc | AB340 x AB337 |
| AB340 | thrC::Phyperspank-med erm | This study |
| AB341 | ΔkinC::cm amyE::PsdpABC-lacZ spc | RL4263 x RL4264 |
| AB342 | ΔkinC::cm Δmed::erm amyE::PsdpABC-lacZ spc | AB142 x AB341 |
| AB343 | ΔkinC::cm thrC::Phyperspank-med erm amyE::PsdpABC-lacZ spc | AB340 x AB341 |
| AB349b | Δmed::erm | This study |
| AB351b | ΔkinD::tet Δmed::erm | AB349 x RL4552 |
| AB392 | amyE::Phyperspank-medspc | This study |
| AB396b | amyE::Phyperspank-med spc | This study |
| AB398b | ΔkinD::tet amyE::Phyperspank-med spc | AB392 x RL4552 |
| AB399b | ΔkinC::cm | RL4263 x 3610 |
| AB400b | ΔkinC::cm Δmed::erm | AB399 x AB142 |
| AB401b | ΔkinC::cm ΔkinD::tet | AB399 x RL4552 |
| AB402b | ΔkinC::cm amyE::Phyperspank-med spc | AB399 x AB392 |
| AB405 | thrC::PxylA-kinD* erm | This study |
| AB413b | Δeps::tet ΔtasA::kan Δmed::erm | AB142 x AB498 |
| AB414b | Δeps::tet ΔtasA::kan ΔkinD::erm | AB535 x AB498 |
| AB415b | Δeps::tet ΔtasA::kan amyE::Phyperspank-med spc | AB392 x AB498 |
| AB430 | ywrK::amyE::Phyperspank-med spc amyE::PsdpABC-lacZ cm | AB392 x RL2886 |
| AB432 | thrC::PxylA-kinD* erm amyE::PsdpABC-lacZ cm | AB405 x RL2886 |
| AB433 | Δmed::tet thrC::PxylA-kinD* erm amyE::PsdpABC-lacZ cm | AB143 x AB432 |
| AB434 | thrC::PxylA-kinD* erm ywrK::amyE::Phyperspank-med spc amyE::PsdpABC-lacZ cm | AB392 x AB432 |
| AB481 | ΔkinA::tet amyE::PsdpABC-lacZ spc | RL4566 x RL4264 |
| AB482 | ΔkinA::tet Δmed::erm amyE::PsdpABC-lacZ spc | AB142 x AB481 |
| AB483 | ΔkinA::tet thrC::Phyperspank-med erm amyE::PsdpABC-lacZ spc | AB340 x AB481 |
| AB484 | ΔkinB::cm amyE::PsdpABC-lacZ spc | RL4261 x RL4264 |
| AB485 | ΔkinB::cm Δmed::erm amyE::PsdpABC-lacZ spc | AB142 x AB484 |
| AB486 | ΔkinB::cm thrC::Phyperspank-med erm amyE::PsdpABC-lacZ spc | AB340 x AB484 |
| AB498b | Δeps::tet ΔtasA::kan | Lab stock |
| AB535b | ΔkinD::erm | This study |
| EH456 | ΔkinD::tet | This study |
| RL2886 | amyE::PsdpABC-lacZ cm | 15 |
| RL3554 | amyE::Pskf-lacZ spc | 11 |
| RL4261 | ΔkinB::cm | Lab stock |
| RL4263 | ΔkinC::cm | Lab stock |
| RL4264 | amyE::PsdpABC-lacZ spc | 15 |
| RL4552b | ΔkinD::tet | EH456 x 3610 |
| RL4566 | ΔkinA::tet | Lab stock |
a
All strains are isogenic with PY79 unless otherwise marked.
b
Strain is isogenic with 3610.
c
The x indicates that DNA from the first strain was introduced into the second strain.