Fig. 2.

Effect of cleavage site mutations on the kinetics of EspPΔ1 passenger domain translocation and cleavage. AD202 (MC4100 ompT::kan) producing the indicated mutants was pulse-labeled and subjected to a chase of up to 10 min (A) or 30 min (B) after the addition of IPTG, and PK was added to half of each sample. Immunoprecipitations were then conducted using an antiserum directed against a C-terminal EspP peptide. Proteins were resolved by SDS-PAGE and visualized using a Fuji BAS-2500 phosphorimager.