Table 1.

Sequences of oligonucleotides used for site-directed mutagenesis

Primer	Sequence (5′-3′)a
Dir-V1022A	TTTCTGAACGAGGCCAACAACCTGA
Rev-V1022A	TCAGGTTGTTGGCCTCGTTCAGAAA
Dir-V1022R	TTTCTGAACGAGCGCAACAACCTGA
Rev-V1022R	TCAGGTTGTTGCGCTCGTTCAGAAA
Dir-N1023A	TGAACGAGGTCGCCAACCTGAACAAA
Rev-N1023A	TTTGTTCAGGTTGGCGACCTCGTTCA
Dir-L1025A	AGTCAACAACGCGAACAAACGTAT
Rev-L1025A	ATACGTTTGTTCGCGTTGTTGACT
Dir-L1025R	AGTCAACAACCGGAACAAACGTAT
Rev-L1025R	ATACGTTTGTTCCGGTTGTTGACT
Dir-K1027A	ACAACCTGAACGCACGTATGGGT
Rev-K1027A	ACCCATACGTGCGTTCAGGTTGT
Dir-R1028A	CAACCTGAACAAAGCTATGGGTGACCT
Rev-R1028A	AGGTCACCCATAGCTTTGTTCAGGTTG
Dir-R1028K	CAACCTGAACAAAAAAATGGGTGACCT
Rev-R1028K	AGGTCACCCATTTTTTTGTTCAGGTTG
Dir-G1030A	CAAACGTATGGCTGACCTGCGT
Rev-G1030A	ACGCAGGTCAGCCATACGTTTG
Dir-G1030R	CAAACGTATGCGTGACCTGCGT
Rev-G1030R	ACGCAGGTCACGCATACGTTTG
Dir-L1032A	ATGGGTGACGCGCGTGATATC
Rev-L1032A	GATATCACGCGCGTCACCCAT
Dir-L1032R	GATATCACGCCGGTCACCCAT
Rev-L1032R	ATGGGTGACCGGCGTGATATC

^aThe mutated nucleotides are in boldface.