Table 2.
CviR DNA binding affinities
| Oligonucleotide sequencea |
Kd (nM) |
|
|---|---|---|
| Mean | SD | |
| CCGCCGTGACCCTTGGAACAGTATCC | >10,000 | |
| CCGCCCAGACCCTTGGAACAGTATCC | >10,000 | |
| CCGCCCTCACCCTTGGAACAGTATCC | >10,000 | |
| CCGCCCTGTCCCTTGGAACAGTATCC | 547 | 110 |
| CCGCCCTGAGCCTTGGAACAGTATCC | 5,836 | 2,805 |
| CCGCCCTGACGCTTGGAACAGTATCC | 7,214 | 2,576 |
| CCGCCCTGACCGTTGGAACAGTATCC | 939 | 219 |
| CCGCCCTGACCCGTGGAACAGTATCC | 1,034 | 303 |
| CCGCCCTGACCCTGGGAACAGTATCC | 1,369 | 224 |
| CCGCCCTGACCCTTGGAACAGTATCC | 975 | 228 |
| CCGCCCTGACCCTTGCAACAGTATCC | >10,000 | |
| CCGCCCTGACCCTTGGTACAGTATCC | 538 | 30 |
| CCGCCCTGACCCTTGGATCAGTATCC | 1,505 | 281 |
| CCGCCCTGACCCTTGGAAGAGTATCC | >10,000 | |
| CCGCCCTGACCCTTGGAACTGTATCC | >10,000 | |
| CCGCCCTGACCCTTGGAACACTATCC | 7,451 | 2,976 |
a
Underlining indicates a mutated nucleotide.