Fig. 1.

WTA-dependent phage infection of S. aureus. Phage lysates from serogroup A (φ47, φSa2mw), serogroup F (φ13, φ77), and serogroup D phages (φK and φ812) were spotted onto lawns of wild-type (w.t.) or cell wall mutant S. aureus RN4220. Macroplaque formation indicates successful adsorption and infection by phages. The bacterial strains used include a ΔtagO mutant (deficient in wall teichoic acid), mutant strains K6 (no GlcNAc modification of wall teichoic acid) and 4S5 (no lipoteichoic acids), and ctagO and cK6, which are tagO- and tarM-complemented strains, respectively, and produce wild-type WTA.