Fig. 5.

The mutant SFM04 is fully segregated. (A) PCR analysis of 4 different colonies (1 to 4) transformed with the vector pSPO1342 (see Materials and Methods) and the wild-type Synechocystis sp. strain PCC 6803 (WT). A control using water as a template (C⁻) is also shown. The oligonucleotides SlexAF2 and slr1735R2 (Fig. 2) were used to prime the reaction. Different sizes of the 1-kb marker from Fermentas are shown on the left. (B) Western blot analyses of protein samples obtained from transformants 1 to 4 and wild-type cells using an anti-GFP antibody (top) and the anti-LexA antibody (bottom) are shown. The molecular masses of the Fermentas protein marker are indicated on the left.