Table 1.

Oligonucleotides used in this study

Primer	Sequence (5′→3′)a	Purpose	Reference
SlexAF	GGATCCGAACCTCTCACCCGAGCCCAAAAAG	Probe for Northern blotting	34
SlexAR	AAGCTTCTAAACTCCCTGGAAATTGCGC		34
SrnpBF	TTCTGTTCCAGGATGCGAGGCA	Probe for Northern blotting	2
SrnpBR	GAGAGTTAGGGAGGGAGTTG		2
3′RACE adapter	GCGAGCACAGAATTAATACGACTCACTATAGGT12CA	3′ RACE (RT)	This work
3′RACE outer	GCGAGCACAGAATTAATACGACT	3′ RACE (1st PCR)	This work
3′RACE inner	GAATTAATACGACTCACTATAGG	3′ RACE (nested PCR)	This work
SlexA-3′RACE1	CAAAGGCGGTGAACTGGTGGAA	3′ RACE (1st PCR)	This work
SlexA-3′RACE2	TGCTATCAAACGCTATTACCAAGA	3′ RACE (nested PCR)	This work
slr1735-3′RACE1	TGATAACTACCCAGACCAACT	3′ RACE (1st PCR)	This work
slr1735-3′RACE2	AGTATCCAATCGGGTCTTTTTCT	3′ RACE (nested PCR)	This work
SlexAF-loc1	GGATCCGAACCTCTCACCCGAGCCCAAAAAG	Fragment 1 (LexA::GFP fusion)	This work
SlexAR-loc1	AAACTGCAGAACTCCCTGGAAATTGCGCCAA		This work
SlexAF-loc2	AAAACGCGTCCCGGCATTACCGGGAACAGCTCAAAAA	Fragment 2 (LexA::GFP fusion)	This work
SlexAR-loc2	AAAAAGCTTATGACTTCCCCCACTGCTCCGTTAA		This work
gfpF	AAACTGCAGTCTAGACTTGAAATGAGCAAGGGCGAGGAG	Fragment 3 (LexA::GFP fusion)	This work
gfpR	AAAACGCGTTTATTACTTGTACAGCTCGTCCAT		This work
pUC4KF	ACGCGTTGAGGTCTGCCTCGTGAAGAA	Fragment 4 (LexA::GFP fusion)	34
pUC4KR	ACGCGTAAAGCCACGTTGTGTCTCAAA		34
SlexAF2	TACTCAAAGCCTCCAACAACAAA	Test chromosome segregation	This work
slr1735R2	ATGCAGTTTGCCCGGGAAGTAT		This work
ShoxprF	GCAATTGGGGTTGCGACTAT	DNA affinity assays	33
ShoxprR	CCTCCACAATCTTGCCCACAATAA		33

^aThe underlined nucleotides correspond to restriction sites.