Table 2.

Oligonucleotide primers used in these studies

Primera	Sequence (5′–3′)b	Purpose	Reference or source
hk1-F	CGTCAATTTATTTTCTAAGGATATTTTC	qRT-PCR	This work
hk1-R	TGCTTCGTCTTCAATTTCACT	qRT-PCR	This work
rrp1-F	AAGGTGCTTACGAGATTGAG	qRT-PCR	This work
rrp1-R	TCTGTGGAACTTCTTGAACTAA	qRT-PCR	This work
ups.hk1-5′	GGGTCCTGGAAGAATACCAGGTTG	Cloning and mutagenesis	This work
dwns.hk1-3′	GTGGGGAGAATCATCCACAATTAA	Cloning and mutagenesis	This work
hk1-KO-5′	CCCATTCAACATTTTTATCCAATTTT	Confirm insertion within hk1	This work
hk1-KO-3′	TGGACCAGCATCATCATTGCTTAGGTCTTTTG	Confirm insertion within hk1	This work
hk1-KO junc-5′	AGGTTAAAAAACGTTAACACCAT	Confirm complementation	This work
flgB-5′	GCGCCATGGTACCCGAGCTTCAAGGAAGA	Construction pSP1G	This work
gent-3′	GCGCCATGGTTAGGTGGCGGTACTTGGG	Construction pSP1G	This work
Hk1 compl-5′	GCGGGATCCGGGTCCTGGAAGAATACCAG	Complementation	This work
Hk1 compl-3′	GCGCTGCAGTTCCACTGCTAATATCTCTTATT	Complementation	This work
flaB-F	CTTTTCTCTGGTGAGGGAGCTC	qRT-PCR, qPCR	53
flaB-R	GCTCCTTCCTGTTGAACACCC	qRT-PCR, qPCR	53
flaB-Probe	CTTGAACCGGTGCAGCCTGAGCA	qRT-PCR, qPCR	53
nidogen-F	CCCCAGCCACAGAATACCAT	qPCR	81
nidogen-R	AAAGGCGCTACTGAGCCGA	qPCR	81
nidogen-probe	CCGGAACCTTCCCACCCAGC	qPCR	81

^aF, forward; R, reverse.

^bRestriction sites are in boldface.