Fig. 7.

3XFT-CopB2 association with inclusions requires chlamydial development. HeLa cells were transfected with p3XFT-CopB2 12 h prior to infection with C. trachomatis L2. Chloramphenicol (E) or INP007 (F) was added to selected cultures at 6 h or 8 h postinfection, respectively. Cultures were processed for microscopy at 8 h (A), 14 h (B), 18 h (C), or 22 h (D to F) postinfection. 3XFT-CopB2 was detected using anti-FLAG (green), and chlamydiae were detected using MOMP-specific antibodies (red). Proteins were visualized by probing with appropriate secondary antibodies and viewed using confocal laser scanning microscopy. Arrows designate colocalization of 3XFT-CopB2 with chlamydial inclusions. Scale bar, 5 μm.