Fig. 8.

C-terminal domains of CopB2 are required for association with inclusion membranes. Full-length CopB2 (A) or constructs carrying in-frame deletions of residues 170 to 196 (B), 276 to 311 (C), or 470 to 506 (D) were expressed in C. trachomatis-infected HeLa cells for 18 h posttransfection. Flag-tagged CopB2 chimeras were detected using anti-FLAG (green), and chlamydiae were detected using MOMP-specific antibodies (red). Proteins were visualized by probing with appropriate secondary antibodies and viewed using confocal laser scanning microscopy. Arrows designate colocalization of CopB2 with chlamydial inclusions. Scale bar, 5 μm.