Fig. 3.
Whole-cell binding of anti-nHgbAI Abs. (A) The reactivities of the Abs from individual pigs actively immunized with nHgbAI (pigs 1 to 4; anti-nHgbAI 2) to 4 different H. ducreyi strains (see the legend for Fig. 3B) were tested in a whole-cell binding ELISA. Antisera from the first nHgbAI/Freund's vaccine trial (anti-nHgbAI 1, pigs 6 to 8) were used positive controls. (B) Pooled nHgbAI antisera used for infusion in each passive immunization experiment and antisera from passively immunized animals (animals C, D, G, and H) were tested in a whole-cell binding assay using the same H. ducreyi strains as shown in Fig. 3A. Pool, anti-nHgbAI 2 pool; NPSp, normal pig serum pool.