Fig. 4.
Reactivities of anti-HgbAI Abs to total cellular proteins from a panel of H. ducreyi strains. The reactivities of pooled anti-nHgbAI 2 to total cellular proteins from H. ducreyi wild-type strains 35000HPhgbAI and DMC111 and several mutants in the 35000HP background were determined in Western blot assays. Anti-nHgbAI 1, pooled swine polyclonal nHgbAI antiserum from a previous nHgbA vaccine trial (pool of antisera from pigs 6, 7, and 8) (1); anti-nHgbAI 2, pooled swine polyclonal nHgbAI antiserum from the current nHgbA vaccine trial (pool of antisera from pigs 1, 2, 3, and 4); anti-rHgbAI, rabbit polyclonal antiserum to denatured rHgbA (28); anti-rD15, rabbit polyclonal antiserum to denatured rD15 (42). The Western blot with anti-rD15 shows equal loading.