Fig. 5.
Anti-nHgbAI Abs bind to the surface of H. ducreyi. The abilities of pooled nHgbAI antisera to bind HgbA at the surface of H. ducreyi strains 35000HPhgbAI, 35000HPhgbAI, and DMC111 were measured in an immunoprecipitation assay. Material obtained from the immunoprecipitation assay was subjected to a 4 to 12% SDS-PAGE and rapid Coomassie blue staining. Pooled antisera used in this assay were obtained from animals immunized 3 times with nHgbAI in Freund's adjuvant, either from a previous study (anti-nHgbAI 1) (1) or obtained in the course of the current study (anti-nHgbAI 2). nHgbAI STD and nHgbAII STD, 0.5 μg of purified nHgbAI or nHgbAII, respectively; TCP, total cellular protein from 1 × 107 CFU; NPSp, normal pig serum pool. Shown is a representative gel from at least 4 different experiments with similar results.