Fig. 4.

Analysis of TCR Vβ repertoires in CFP-10-specific IFN-γ-producing CD4⁺ (B) and CD8⁺ (C) T cells in tubercular pleural fluid. (A) Representative example of flow cytometry of PFMCs. The lymphocyte gating was set with forward and side light scatter. CD4⁺ and CD8⁺ T cells were gated according to the expression of CD3, CD4, and CD8. The expression of 24 TCR Vβ chains by CFP-10-specific CD4⁺ or CD8⁺ PFMCs was then assessed. Different TCR Vβ chains were analyzed by use of eight tubes. In each tube, from A to H, one TCR Vβ antibody was conjugated to FITC, another to PE, and a third to both FITC and PE. PFMC TCR Vβ repertoires following stimulation of cells with CFP-10 for 8 h are indicated for 10 patients with tuberculous pleurisy (TB1 to TB10). Percentages of IFN-γ production by CD4⁺ (B) or CD8⁺ (C) TCR Vβ chains are indicated for each donor.