Fig. 2.

Genome location and distribution of genes encoding CWA proteins. (A) Genome map of S. pseudintermedius ED99 indicating the genomic location of each gene. (B) Distribution of the genes encoding putative CWA proteins among 20 S. pseudintermedius strains, representatives of the closely related S. delphini and S. intermedius, and other staphylococcal species associated with animal skin disease. The diversity of strains is indicated by a phylogenetic tree constructed using the neighbor-joining method, with bootstrap values over 60 indicated; a blue square denotes presence of a gene, and an empty square indicates its absence, based on Southern blot analysis (for spsA to spsO) or PCR amplification (for spsP, spsQ, and spsR).