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. 2011 Aug;79(8):3348–3357. doi: 10.1128/IAI.05124-11

Fig. 4.

Fig. 4.

Application of the P388D1-lacZ screen identifies a candidate virulence mutant. Macrophages infected with wild-type Histoplasma yeast or the 16E1 insertion mutant demonstrate that the 16E1 mutant is attenuated in virulence in macrophages. (A) P388D1-lacZ macrophages were infected with wild-type (WU15) or mutant yeast (OSU10) at MOIs of 1:1 or 3:1 yeast per macrophage. At 7 days postinfection, remaining macrophages were quantified by β-galactosidase activity, and results are plotted following normalization to results for uninfected wells. (B) Murine bone marrow-derived macrophages (BMDM) were infected with wild-type (OSU25) or mutant (OSU74) yeast at MOIs of 10:1 and 20:1 for 48 h. The number of intact macrophages was determined by measurement of the remaining macrophage DNA using PicoGreen fluorescence. Values are presented as averages ± SD from three wells and are representative of three independent experiments. Statistical differences from wild-type results (*, P < 0.05; ***, P < 0.001) were determined by Student's t test.