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Primers used for quantitative reverse transcriptase PCR amplification
| mRNA target | 5′ primer sequence | 3′ primer sequence | Product length (bp) |
|---|---|---|---|
| β-Actin | CAGGCACCAGGGCGTGATG | GTACATGGCTGGGGTGTTGA | 286 |
| MAPK8 (JNK) | CAGTCAGGCAAGGGATTTGT | TGATGATGGATGCTGAGAGC | 270 |
| ROCK1 | GGTTAGGGCGAAATGGTGTA | AGTTGATTGCCAACGAAAGC | 207 |
| COL5A1 | GGCTGTGCTACCAAGAAAGG | GAGGTCACGAGGTTGCTCTC | 197 |
| 18S rRNA (normalization control) | CGGCTACCACATCCAAGGAA | GCTGGAATTACCGCGGCT | 186 |
