Skip to main content
. 2011 Aug;79(8):3249–3261. doi: 10.1128/IAI.05122-11

Table 3.

Genes belonging to selected significantly differentially expressed KEGG pathways in Ea.hy926 cells infected with p66+ versus Δp66 B. burgdorferi bacteriaa

Pathway Gene name(s)b Protein encoded Fold change comparing the followingc:
 Predicted effect(s) or note(s)d
p66+ and uninfected Δp66 and uninfected p66+ and Δp66
Regulation of actin cytoskeleton/focal adhesion ARHGEF1A Rho guanine nucleotide exchange factor (GEF) 1 −1.6 NS −1.5 Decreased actin polymerization +P66
GSNA Gelsolin −1.8 NS −2.0 Decreased actin polymerization +P66
ACTN4A Actinin, alpha 4 NS −1.6 1.5 Decreased actin polymerization −P66
PI3K-R2, PI3K-R3, and PI3K-CGA,L Phosphoinositide-3-kinases NS 2.2 −2.0 Increased inflammation −P66, decreased actin polymerization, increased vessel permeability +P66
TAOK2A TAO kinase 2, PSK2 −1.7 NS −1.9 Decreased MAPK p38 signaling, decreased inflammatory cytokine induction +P66
RAC1L Rho family, small GTP-binding protein Rac1 NS 2.76 −2.01 Increased actin polymerization, −P66, decreased JNK activation, decreased cortical F actin, increased vessel permeability +P66
ROCK1L* Rho-associated, coiled-coil containing protein kinase 1 −1.63 NS −2.20 Increased actin polymerization −P66
ACTBL* β-Actin NS 2.63 −2.41 Increased actin polymerization −P66
PPP1R12AL Protein phosphatase 1, regulatory (inhibitor) subunit 12A −1.52 2.03 −1.88 Increased actin polymerization −P66
MYL6L Myosin, light chain 6, alkali, smooth muscle and nonmuscle NS 2.06 −1.87 Increased actin polymerization −P66
PTK2L Protein tyrosine kinase 2, FAK NS 1.97 −2.4 Increased actin polymerization −P66
PRKCB1L Protein kinase C, beta 1 NS NS −1.82 Decreased MAPK signaling, focal adhesion, immune response +P66
VAV2L Rho family GEF NS 2.26 −1.77 Increased Rho activation −P66
Jak-STAT, MAPK, VEGF, and other signaling pathways STAT1L, STAT4L, STAT6A Signal transducers and activators of transcription −1.7 (1 and 6), 5.81 (4) NS (1 and 6), −2.18 (4) −2.0 (1 and 6), 12.66 (4) Decreased immune response +P66 for STAT1 and STAT6, opposite for STAT4
IL11RAA Interleukin 11 receptor, alpha NS 1.9 −2.2 Increased immune response −P66
PI3K-R2, PI3K-R3, and PI3K-CGA,L Phosphoinositide-3-kinases NS 2.2 −2.0 Increased inflammation −P66, decreased actin polymerization +P66, increased vessel permeability +P66
TAOK2A TAO kinase 2, PSK2 −1.7 NS −1.9 Decreased MAPK p38 signaling, decreased inflammatory cytokine induction +P66
PLA2-G2A, PLA2-G2E, and PLA2-G5A,L Phospholipase A2, groups II and V 1.7 −1.6 2.7 Increased inflammation +P66, decreased inflammation −P66
RAC1L Rho family, small GTP-binding protein Rac1 NS 2.76 −2.01 Increased actin polymerization, −P66, decreased JNK activation, decreased cortical F actin, increased vessel permeability +P66
MAPK8, JNKL* Mitogen-activated protein kinase 8; Jun N-terminal kinase −1.56 NS −2.05 Decreased JNK signaling, inflammation +P66
MAPK14, p38L Mitogen-activated protein kinase 10 NS 1.70 −2.3 Increased inflammation −P66
RapGEF2L Rap guanine nucleotide exchange factor (GEF) 2 1.55 2.69 −1.74 Decreased MAPK signaling +P66
PRKCB1L Protein kinase C, beta 1 NS NS −1.82 Decreased MAPK signaling, focal adhesion, immune response +P66
CACNG1, CACNG4, and CACNG5L Calcium channel γ subunits NS 1.80 −1.84 Similar changes for all three; increased Ca2+ signaling −P66
CACNB3L Calcium channel β subunit NS 3.16 −3.72 Increased Ca2+ signaling −P66
HSP72L Heat shock protein 72 NS 1.99 −1.92 Increased proinflammatory cytokine production −P66, increased protection against stress −P66
Defense response, response to wounding, stress NOS2AA Nitric oxide synthase 2A (inducible) −1.9 2.6 −5.0 Decreased immune response +P66, increased immune response −P66
    response TNIP1A TNFAIP3-interacting protein 1 1.8 NS 1.8 Decreased immune response +P66
F2RL3A Coagulation factor II receptor-like 3 −1.5 1.6 −2.5 Decreased immune response +P66, increased immune response −P66
CEACAM1 A Carcinoembryonic antigen-related cell adhesion molecule 1 NS −1.8 1.9 Decreased cell adhesion, integrin signaling −P66
PLA2-G2A, PLA2-G2E, and PLA2-G5A,L Phospholipase A2, groups II and V 1.7 −1.6 2.7 Increased inflammation +P66, decreased vessel permeability +P66, decreased inflammation −P66
PRKCB1L Protein kinase C, beta 1 NS NS −1.82 Decreased MAPK signaling, focal adhesion, immune response +P66, increased vessel permeability +P66
HSP72L Heat shock protein 72 NS 1.99 −1.92 Increased proinflammatory cytokine production −P66, increased protection against stress −P66
PI3K-R2, PI3K-R3, and PI3KCGA,L Phosphoinositide-3-kinases NS 2.2 −2.0 Increased inflammation −P66, decreased actin polymerization +P66, increased vessel permeability +P66
MAPK14, p38L Mitogen-activated protein kinase 10 NS 1.70 −2.3 Increased inflammation −P66
IL11RAA Interleukin 11 receptor, alpha NS 1.9 −2.5 Increased immune response −P66
MAPK8, JNKL* Mitogen-activated protein kinase 8; Jun N-terminal kinase −1.56 NS −2.05 Decreased JNK signaling, inflammation +P66
a

Ea.hy926 cells were infected with B. burgdorferi at an MOI of 10 or left uninfected. Bacterial cell-host cell contact was not facilitated by centrifugation. For genes represented more than once on each array, all the data were averaged. Genes listed in one pathway are included again in different pathways when appropriate.

b

Adherent cells are indicated by a superscript A letter, and lifted cells are indicated by a superscript L letter. Cells that are both adherent and lifted cells are indicated by both superscript A and L letters. Some genes were validated using quantitative reverse transcriptase PCR (qRT-PCR); these genes are indicated with an asterisk, and the qRT-PCR values are shown in parentheses.

c

The fold change for value for the following three comparisons is shown: p66+ B. burgdorferi-infected cells and uninfected cells (p66+ and uninfected), Δp66 B. burgdorferi-infected cells and uninfected cells (Δp66 and uninfected), and p66+ B. burgdorferi-infected cells and Δp66 B. burgdorferi-infected cells (p66+ and Δp66). The data shown are the average fold changes for three independent experiments at one or more time points. NS, not significant. Parenthetical 1, 4, and 6 refer to STAT1 to STAT6, respectively.

d

The predicted effect or note in the presence of P66 (+P66) or in the absence of P66 (−P66) is shown.