Table 1.
List of bacterial strains
| Strain | Genotype or description | Reference or source |
|---|---|---|
| Clostridium difficile | ||
| JIR8094 | Spontaneous erythromycin-sensitive derivative of 630 | 41 |
| TCD31 | JIR8094 prsW1::ltrB::ermB | |
| Escherichia coli | ||
| Omnimax-2 T1R | F′ [proAB+lacIqlacZΔM15 Tn10(Tetr) Δ(ccdAB)] mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 Δ(lacZYA-argF)U169endA1recA1supE44 thi-1gyrA96relA1tonA panD | Invitrogen |
| HB101/pRK24 | F−mcrB mrr hsdS20(rB− mB−)recA13 leuB6 ara-14 proA2 lacY1 galK2 xyl-5 mtl-1 rpsL20 | |
| CDE1408 | Omnimax/pCE263 (pDG1663 + PcsfT-lacZ [mls]) | |
| CDE1419 | Omnimax/pCE268 (pDG1663 + PcsfU-lacZ [mls]) | |
| CDE1440 | Omnimax/pCE286 (pDG1663 + PcsfV-lacZ [mls]) | |
| CDE1446 | Omnimax/pCE292 (pDR111 “HindIII” RfA) | |
| CDE1614 | Omnimax/pJK10-1 (pDR111 + Phs-csfT+ Spt) | |
| CDE1615 | Omnimax/pJK12-E5A (pDR111 + Phs-csfT+ rsiT+ Spt) | |
| CDE1616 | Omnimax/pJK8-C42 (pDR111 + Phs-csfU+ Spt) | |
| CDE1617 | Omnimax/pTHE172-82 (pDR111 + Phs-csfU+ rsiU+ Spt) | |
| CDE1618 | Omnimax/pTHE166-V22 (pDR111 + Phs-csfV+ Spt) | |
| CDE1619 | Omnimax/pTHE200-K41 (pDR111 + Phs-csfV+ rsiV+Spt) | |
| CDE1620 | Omnimax/pTHE1031 (pDR111 + Phs-2×Flag-rsiT+ Spt) | |
| CDE1621 | Omnimax/pTHE1038 (pDR111 + Phs-2×Flag-rsiU+ Spt) | |
| CDE1622 | Omnimax/pTHE1046 (pDP150 + Phs-prsWC. difficile+ mls) | |
| Bacillus subtilis | ||
| PY79 | Protrophic derivative of Bacillus subtilis 168 | 58 |
| CDE1410 | PY79 thrC::PcsfT-lacZ (mls) | |
| CDE1601 | PY79 thrC::PcsfT-lacZ (mls) amyE::Phs-csfT+(Spt) | |
| CDE1602 | PY79 thrC::PcsfT-lacZ (mls) amyE::Phs-csfT+ rsiT+ (Spt) | |
| CDE1603 | PY79 thrC::PcsfT-lacZ (mls) amyE::Phs-csfU+(Spt) | |
| CDE1604 | PY79 thrC::PcsfT-lacZ (mls) amyE::Phs-csfV+(Spt) | |
| CDE1420 | PY79 thrC::PcsfU-lacZ (mls) | |
| CDE1605 | PY79 thrC::PcsfU-lacZ (mls) amyE::Phs-csfU+(Spt) | |
| CDE1606 | PY79 thrC::PcsfU-lacZ (mls) amyE::Phs-csfU+ rsiU+ (Spt) | |
| CDE1607 | PY79 thrC::PcsfU-lacZ (mls) amyE::Phs-csfT+(Spt) | |
| CDE1608 | PY79 thrC::PcsfU-lacZ (mls) amyE::Phs-csfV+(Spt) | |
| CDE1609 | PY79 thrC::PcsfV-lacZ (mls) | |
| CDE1610 | PY79 thrC::PcsfV-lacZ (mls) amyE::Phs-csfV+(Spt) | |
| CDE1611 | PY79 thrC::PcsfV-lacZ (mls) amyE::Phs-csfV+ rsiV+ (Spt) | |
| CDE1612 | PY79 thrC::PcsfV-lacZ (mls) amyE::Phs-csfT+(Spt) | |
| CDE1613 | PY79 thrC::PcsfV-lacZ (mls) amyE::Phs-csfU+(Spt) | |
| CDE1623 | PY79 ΔprsW::kan amyE::Phs-2×Flag-rsfT+ (Spt) | |
| CDE1624 | PY79 ΔprsW::kan amyE::Phs-2×Flag-rsfT+(Spt) thrC::Phs-prsW+(mls) | |
| CDE1625 | PY79 ΔprsW::kan amyE::Phs-2×Flag-rsfU+(Spt) | |
| CDE1626 | PY79 ΔprsW::kan amyE::Phs-2×Flag-rsfU+(Spt) thrC::Phs-prsW+(mls) |