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. 2011 Aug;79(8):3064–3073. doi: 10.1128/IAI.05148-11

Fig. 2.

Fig. 2.

Expression of T3SS-3 and T6SS-1 genes in B. pseudomallei wild-type strain (KHW) and regulator mutants. (A) Transcript levels of regulators and T3SS-3 genes in ΔbspR, ΔbprP, and ΔbsaN strains during infection of RAW 264.7 cells were measured by real-time PCR and normalized to those of KHW. (B) Transcript levels of regulators and T6SS-1 genes in KHW, ΔbprC, and ΔvirAG strains in RPMI medium (Medium) and during infection of RAW 264.7 cells (RAW cells). (C) The expression of tssA in the ΔbprC mutant could be complemented by providing bprC in trans on a plasmid (pMLbprC). (D) The expression of the T6SS-1 genes in the virAG mutant could be restored by virAG on a plasmid (pMLvirAG) but not by an individual gene (pMLvirA or pMLvirG).