Table 3.

List of PCR primers used for constructing plasmids

Primer name	Sequence (5′–3′)a	PCR amplification
BprC for	AAGCTTACGTGTTGCGCGACATTC	Forward primer for bprC ORFb
BprC rev	AAGCTTCGGAATCGACATTCATCGT	Reverse primer for bprC ORF
VirA for	GGTACCTATTCGCCGACCTCTGAGAC	Forward primer for virA, virAG ORFs
VirA rev	AAGCTTCGACAGGTAATTGCAAAGCA	Reverse primer for virA ORF
VirG for	GGTACCGGCGCAGTACAGGAGAGGAC	Forward primer for virG ORF
VirG rev	AAGCTTCCGACGAGCAAATCCTTG	Reverse primer for virA, virAG ORFs
PtssA for	TTGAATTCATGGAATTTCCGTGCATAGG	Forward primer for tssA promoter and ORF
PtssA rev	TTGAATTCAAGCTTCCATGGTCGTCTGTTCC	Reverse primer for tssA promoter and ORF
Phcp1 for	GAATTCAAGCTCGAAAAGCGCTAGG	Forward primer for hcp1 promoter and ORF
Phcp1 rev	AAGCTTATCAGCCATTCGTCCAGTTT	Reverse primer for hcp1 promoter and ORF
TssAB-up for	GAATTCGATGCACATCGACAGGATC	Forward primer for upstream of tssAB
TssAB-up rev	GCGATAGACGATGTTGACC	Reverse primer for upstream of tssAB
TssAB-dn for	ATCCAGAACAAGATCCCGA	Forward primer for downstream of tssAB
TssAB-dn rev	AAGCTTTTCCAACGAGTATCCCGAA	Reverse primer for downstream of tssAB

^aRestriction sites are underlined.

^bORF, open reading frame.