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Immunoblot analysis of PilW (top) and PilQ (bottom) in a pilW/pilWWT strain in the presence of increasing concentrations of IPTG. This strain is a pilW mutant complemented by the ectopic integration of a wild-type pilW allele placed under the control of an IPTG-inducible promoter. The WT strain and the pilW mutant were included as controls. Equal amounts of whole-cell protein extracts were loaded in each lane. Monomers and multimers of PilQ are indicated by arrows.
