Fig. 2.

Piliation as assessed by immunofluorescence microscopy. Tfp (green) were labeled with the monoclonal antibody 20D9, which is specific for N. meningitidis 8013 filaments, while the bacteria (red) were stained with ethidium bromide. For the sake of clarity, only a few representative PilW variants constructed in this study are shown. Results for all of the strains are summarized in Table 2. Bar, 5 μm.