Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Immunoblot analysis of PilW production/stability (top) and PilQ multimerization (bottom) in PilW variants constructed in this study. Strains were grown overnight on plates containing 12.5 μM IPTG, unless otherwise indicated. Whole-cell lysates were prepared and quantified, and equal amounts of total protein were loaded in each lane. The WT strain and the pilW mutant were included as controls. Monomers and multimers of PilQ are indicated by arrows.
