Table 2.
Summary of the Tfp-linked phenotypic properties in PilW variants assessed during this studya
| Strain genotype | Tfp biology featureb |
|||||
|---|---|---|---|---|---|---|
| Piliation | Aggregation | Twitching motility | Adherence to HUVEC | Competence | PilQ multimers | |
| WT | + | + | + | + | + | + |
| pilW | − | − | ND | − | − | − |
| pilW/pilWWT | + | + | + | + | + | + |
| pilW/pilW(C20G) | +/− | +/− | ND | − | +/− | − |
| pilW/pilW(C20G)c | +/− | + | + | − | +/− | +/− |
| pilW/pilW(C115G) | +/− | − | ND | − | +/− | − |
| pilW/pilW(C150G) | +/− | − | ND | − | +/− | − |
| pilW/pilW(N108A) | + | + | + | + | + | + |
| pilW/pilW(N109A) | + | + | + | + | + | + |
| pilW/pilW(Y137A) | + | + | + | + | + | + |
| pilW/pilW(N146A) | + | + | + | + | + | + |
| pilW/pilW(N108A,Y137A) | + | + | + | +/− | + | + |
| pilW/pilW(N108A,N146A) | + | + | + | + | + | + |
| pilW/pilW(N109A,Y137A) | + | + | + | +/− | + | + |
| pilW/pilW(N109A,N146A) | + | + | + | + | + | + |
| pilW/pilW(Y137A,N146A) | + | + | + | +/− | + | + |
a
The WT strain and the pilW mutant are included as positive and negative controls, respectively. Experiments were performed in the presence of 12.5 μM IPTG, except where indicated.
b
Phenotypes are scored as follows: +, WT level; −, pilW mutant level; +/−, partial restoration of WT phenotype; ND, not determined.
c
Experiments were performed in the presence of 100 μM IPTG.