Table 3.
Sequence comparison of ORFs detected in the 3,122-bp fragment sequenced from phage Φ125 with previously published sequences
| ORF | Position (bp) | Size (bp) | Identified gene | Description | Most similar sequencea | % Similarityb | Reference |
|---|---|---|---|---|---|---|---|
| ORF1 | 76-852 | 777 | cdtA-V | Cytolethal distending toxin V | gb|AY365043.1| | 100 | 4 |
| A subunit Binding protein for delivering CdtB into target cells | CdtA-V in E. colistrain 5249/01 | ||||||
| ORF2 | 849-1658 | 810 | cdtB-V | Cytolethal distending toxin V | gb|AY365043.1| | 100 | 4 |
| B subunit catalytic subunit | CdtA-V in E. colistrain 5249/01 | ||||||
| ORF3 | 1673-2218 | 546 | cdtB-V | Cytolethal distending toxin | gb|AY365043.1| | 100 | 4 |
| C subunit binding proteins for delivering CdtB into target cells | CdtA-V in E. coli strain 5249/01 | ||||||
| ORF4 | 2329-3211 (truncated) | 882 | gpA (fragment) | Phage protein A; DNA replication; makes site-specific nick at origin of replication | gb|AF063097.1| bacteriophage P2; complete genome; gene “A” | 97 | 24 |
a
The GenBank (gb) accession numbers are indicated.
b
That is, the percent similarity to the most similar sequence in the previous column.