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. 2011 Aug;79(8):3397–3406. doi: 10.1128/IAI.01080-10

Fig. 5.

Fig. 5.

Oral vaccination (vax) with attenuated parasites stimulates T. cruzi-specific CD8+ T cells. (A) Variable degrees of T cell stimulation result from oral vaccination. CD44 expression was assessed 2 weeks after final vaccination on CD8+ T cells in PBMC. Of 10 vaccinated mice, 3 were designated LR and 7 were designated HR. Flow plots are gated on CD8+ T cells, and numbers and gates indicate the average percentage of CD44hi cells in each group. (B) Oral vaccination generates activated T. cruzi-specific CD8+ T cells. Flow plots are from an individual mouse chosen from HR group to screen for TSKb20+ responses and CD127 downregulation. All plots are gated on CD8+ T cells, and numbers indicate the percentage of CD8+ T cells in a gate or quadrant.