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Superose 6 prep-grade gel filtration profile of sonicated PMA conjugated to type a flagellin. (A) Fractions were assayed for protein by the Bradford assay (595 nm) and for uronic acid by the carbazole assay (525 nm). (B) ELISA for inhibition based on the competitive inhibition by PMA or PMA-FLA of the binding of the anti-PMA antibodies to PMA.
