Table 2.
Estimated dilution of serum needed to mediate opsonophagocytic killing of 50% (EC50) of the target bacterial strain
| Target strain | Titer (95% confidence interval) mediating EC50 in antiserum raised to: |
||
|---|---|---|---|
| Flagellin | PMA | PMA-FLA | |
| PAK (type a) | 10 (6-17) | 14 (6-32) | 5 (2-13) |
| N6 (type a) | <4a | 7 (3-16) | <4 |
| N13 (type a) | <4 | <4 | <4 |
| PA01 (type b) | 6 (3-12) | 16 (9-28) | <4 |
| FRD1 | N/A | 545 (273-1,088) | 382 (275-529) |
| 2192 | N/A | 34 (21-56) | 36 (18-70) |
| 8050 | N/A | 15 (11-22) | 13 (8-22) |
| FRD1 ΔalgD | N/A | <4 | <4 |
a
Opsonic killing of <30% in the 1:4 serum dilution was considered to be within the range of the controls lacking an essential component needed for opsonization and/or killing, and thus titers are reported as less than 4.