Table 1.
Plasmids and strains used in this study
| Plasmid or strain | Descriptiona | Source or reference |
|---|---|---|
| Plasmids | ||
| pCR2.1-TOPO | PCR cloning vector | Invitrogen |
| pML102 | Customized donor plasmid for in vitro mutagenesis | 74 |
| pBVS2 | Borrelial shuttle confers Kanr | 78 |
| pBBE22 | Borrelial shuttle vector confers Kanr derived from pBSV2 with BBE22 region of lp25 | 61 |
| pSVR1 | 1.9 kb of lp54 spanning BBA34 in pCR2.1-TOPO | This study |
| pSVR2 | SVR1 with 1.6-kb insertion of PflgB-aadA at the nucleotide 154 bp from the 5′ end of BBA34 | This study |
| pSVR3 | Borrelial shuttle vector pBBE22 with functional copy of bba34 | This study |
| B. burgdorferi strains | ||
| MSK5 | B31 isolate with all infection-associated plasmids | 44 |
| ML23 | B31, lp25-negative, noninfectious clonal isolate | 44 |
| ML23/pBBE22 (wt) | ML23 with pBBE22 (BBE22+), Kanr | 74 |
| SVR2 | bba34 mutant in ML23; lp25−, Strr | This study |
| SVR2/pBBE22 (mt) | SVR2 with pBBE22; Kanr; Strr | This study |
| SVR3 (ct) | SVR2 with pSVR3 (complementation plasmid, pBBE22/bba34+) | This study |
a
Strr, streptomycin resistance; Kanr, kanamycin resistance.