Table 3.
Virulence of B. anthracis Ames, B. anthracis Sterne, and B. cereus G9241 wild-type and plasmid-cured derivatives in mice
| Mouse strain | Bacterial strain | Subcutaneous LD50a | Source(s) | Intranasal LD50a | Source(s) |
|---|---|---|---|---|---|
| A/J | B. anthracis | ||||
| Ames (pXO1+/pXO2+) | 0.7 | 21 | 3.8 | 21 | |
| Sterne (pXO1+/pXO2−) | 3.2 | 5, 33, 41 | 4.8 | 5, 33 | |
| B. cereus G9241 | |||||
| pBCXO1+/pBC218+ | 3.1 (2.8–3.4)b | This study | 5.5c | This study | |
| pBCXO1+/pBC218− | 6.8 (6.5–7.2)b | This study | >7 | This study | |
| pBCXO1−/pBC218+ | >7 | This study | >7 | This study | |
| pBCXO1−/pBC218− | >7 | This study | >7 | This study | |
| C57BL/6J | B. anthracis | ||||
| Ames (pXO1+/pXO2+) | 1.4 | 4, 21 | 4.1 | 21 | |
| Sterne (pXO1+/pXO2−) | 5.9 | 41 | >7 | This study | |
| B. cereus G9241 | |||||
| pBCXO1+/pBC218+ | 3.7 (3.3–4.1)b | This study | 5.8 (5.4–6.2)b | This study |
a
LD50 values are the log10 CFU, with 95% confidence intervals indicated in parentheses.
b
LD50 values and confidence intervals were determined with the probit method.
c
The LD50 value was determined with the Reed-Muench method (32), as these data did not permit analysis by probit.