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. 2011 Aug;31(15):3094–3104. doi: 10.1128/MCB.01449-10

Fig. 2.

Fig. 2.

CTCF and cohesins colocalize at KvDMR1 in MEFs. (A) Schematic showing the Kcnq1/Kcnq1ot1 locus. The transcriptional and methylation statuses of the maternal and paternal chromosomes are represented at the top and bottom of the DNA strand, respectively. The KvDMR1 region is enlarged at the bottom. The regions analyzed in B, C, and D are indicated below the diagram. (B) ChIPs followed by real-time PCR. *, P < 0.05; **, P < 0.01. P values show differences between no-antibody (No-Ab) controls and antibodies against proteins of interest. The data are normalized to the no-antibody controls of each PCR. Error bars indicate standard errors of data from at least three biological replicates. (C and D) Allele-specific analysis of ChIPed DNA by PCR showing binding of CTCF and cohesins in KvDMR1. (C) CTCF BS-1; (D) CTCF BS-2. AcH3 and H3K4me2 are included as controls. B, maternal allele; C, paternal allele. The percentage of protein binding on the B allele over total binding (B plus C) is indicated at the bottom. At least three experiments were performed, and similar results were obtained.