Fig. 4.

Oligomers are converted to large aggregates by HspB1. (A) Atomic force microscopy images of unassembled Aβ and oligomers before (−HspB1) and after (+HspB1) coincubation with HspB1. Each field of view is 2 μm by 2 μm. The color bar on the right indicates the height of the particles. Bar, 500 nm. (B) The AFM images for oligomers without HspB1 and with HspB1 were analyzed for the height (blue) and area (red) of particles. (C) Electron microscopy of oligomers, oligomer coincubated with HspB1, and HspB1. The arrowheads in the middle panel indicate the large aggregates. (D) Kinetics of interaction between Aβ and HspB1 was monitored by light scatter. Light scatter of unassembled or oligomer samples of Aβ (10 μM as monomers) was examined at 350 nm (5-nm band-pass) in the absence (Buffer) or presence of 2 μM BSA or presence of 2 μM HspB1.